A&A Biotechnology
A&A Biotechnology

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MagnifiQ™ 1 ExTerminator instant kit

Automated, magnetic removal of nucleotide dye-terminators kit after DNA cycle sequencing reactions in the strip format. Contains ready-to-use, reagent-filled stripes and all necessary consumables. The strip format enables isolation of a single sample per purification run.
Product Code:SizePriceQty
444A-1V-32 32 isolations
    108,00 €
Manual
444A-1V-160 160 isolations
    484,56 €
Manual

Specification

protocol time~ 23 min
sample typecycle sequencing PCR products
sample sizeup to 2ml
elution volume50 µl
elution solutionultrapure water / deionised formamide
binding capacity10 µg DNA
downstream applications

capillary electrophoresis of:

  • de novo Sanger DNA sequencing;
  • plasmid sequencing
  • microbial species identification
  • single-nucleotide polymorphism (SNP) genotyping
  • CRISPR-Cas9 genome editing analysis

Protocol files

deviceprotocol nameprotocol fileinstallation
Auto-Pure MiniMQ-EXT-MIaabiot.com/protocols/magnifiq/MI/MQ-EXT-MI.txt
  1. Create folder "items" on a USB drive and copy the protocol file to it.
  2. Insert the USB drive into a USB slot in the device.
  3. On a device screen, go to Settings > System > Transfer >Import.
  4. Select the protocol and tap "Import".

Auto-Pure Mini

(QR code)

MQ-EXT-MI
  1. On a device screen, go to Run > >
  2. Scan the QR code with the device's scanner.
Auto-Pure S32MQ_EXT_S32aabiot.com/protocols/magnifiq/S32/MQ_EXT_S32.txt
  1. Create folder "im_export_protocols" on a USB drive and copy the protocol file to it.
  2. Insert the USB drive into a USB slot in the device.
  3. On a device screen, go to Protocols >Import.
  4. Select the protocol and tap "Import".

Description

MagnifiQ™ 1 ExTerminator instant kit is designed for rapid and reliable removal of dye-terminators from the cycle sequencing products prior to the capillary electrophoresis sequencing run. Like previous A&A Biotechnology ExTerminator Kits this product also provides the visual control of the purification process by means of Blue Mix reagent. The purified material is perfectly suitable for resolving the sequencing run on automated DNA sequencers including ABI3100,  SeqStudio and other automated genetic analysers capable of resolving the BigDye terminator chemistries.

The MagnifiQ™ product series is based on the automated isolation of nucleic acids with use of magnetic beads. This method significantly shortens working time and reduces risk of mistakes in comparison to manual methods.

MagnifiQ™ 1 ExTerminator instant kit

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Manual