T4 DNA polymerase

form

solution

concentration

3 U/µl

unit definition

One unit of the enzyme catalyzes the incorporation of

10 nmol of deoxyribonucleotides into a polynucleotide

fraction in 30 min at 37 °C.

applications

• Blunting of dsDNA ends by filling 5'-overhangs or removal of 3'-overhangs.
• Blunting of PCR products containing 3'-dA overhangs (after amplification with Taq DNA polymerase)
• Synthesis and labeling of DNA probes by the replacement reaction.
• Oligonucleotide-directed site-specific mutagenesis.
• Ligation-independent cloning of PCR products.

source

recombinant

storage buffer

100 mM KPO₄, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C