JO-FI™ competent cells
High efficiency competent cells suitable for any type of cloning method and standard plasmid DNA transformation. Stable replication of high-copy plasmids.
| Product Code: | Size | Price | Qty | ||
|---|---|---|---|---|---|
| 1033-20 | 20 pcs |
| Manual | MSDS | |
| 1033-40 | 40 pcs |
|
Advantages of the product
- High transformation efficiency: 5–6 x 10⁹ cfu/µg pUC19 DNA
Specification
- Tetracycline (15 µg/ml) and Streptomycin (25 µg/ml) resistance.
- The lacIq repressor enables tight, IPTG -dependent expression control from the trc, tac and lac promoters.
- The lacZΔM15 deletion allows the white-blue color selection of recombinant clones (LA + X-Gal).
- recA1 gene mutation reduces the occurrence of undesired recombination events during cloning.
- endA1 gene mutation decreases endonuclease I activity. This results in a lower level of DNA degradation, providing faster plasmid DNA transfer.
- mcrA gene mutation allows efficient transformation of methylated genomic DNA.
- hsdR gene mutation allows efficient transformation of unmethylated DNA after PCR amplification.
